Optimization of triterpensaponin extraction from Bacopa monnieri (l.) Wettst by enzyme cellulase

Nguyen Thi Huong Lan * , Phung Thi Ngoc Huyen , Nguyen Thi Thu Thao , Truong Trong Nguyen , & Hoang Thi Truc Quynh

* Correspondence: Nguyen Thi Huong Lan (email: quynhhtt@cntp.edu.vn)

Article Sidebar

PDF
Received: 07 Nov 2018
Revised: 17 Dec 2018
Accepted: 17 Dec 2018
Published: 15 Jan 2019
DOI: 10.58810/vhujs.6.3.2019.6351
Views
0
Downloads
0
How to Cite
Nguyễn, L. . T. H., Phùng, H. T. N., Nguyễn, T. T. T., Trương, N. T., & Hoàng, Q. T. T. (2019). Optimization of triterpensaponin extraction from Bacopa monnieri (l.) Wettst by enzyme cellulase. Van Hien University Journal of Science 6(3), 120-131.
Issue
Volume 6 - Issue 3 (2019)
Section
Công nghệ thực phẩm

Main Article Content

Abstract

Bacopa monnieri (L.) Wettst is widely distributed in tropical and subtropical areas. In this study, the enzyme assisted extraction, was extracted of triterpensaponin from Bacopa monnieri. Screening factors include the ratio of raw materials: solvent; pH; temperature, extracting time and enzyme concentration. The optimum conditions for extracting triterpensaponin from Bacopa monnieri with a cellulase enzyme modeled as Plackett-Burman model showed a treatment temperature of 50 °C, a ratio of 1: 21 (w/v), enzyme concentration 0.81% (v/w), pH 5.3 and 37 minutes extraction time achieved the highest triterpensaponin content of 2.63 (% g/g CK). (1.3 times higher than that of control no enzyme). Thus, the enzyme-assisted extraction method is an effective method, which improves the extraction efficiency of triterpensaponins from Bacopa monnieri.
Keywords: Bacopa monnieri (L.) Wettst, cellulase enzyme, content of triterpensaponin, response surface model, extraction.

Article Details

References

Chaturvedi S., Hemamalini R. and Khare S.K. (2012). Effect of processing conditions on saponin content andantioxidant activity of Indian varieties of soybean (Glycine max Linn.). Annals of Phytomedicine, 1(1), pp. 62-68.

Choon Y. C., Hanaa S. and Rabiha S. (2014). Extraction and quantification of saponins: A review. Food Research International, (59), pp.16-40.

Dar A. and Channa S. (1999). Calcium antagonistic activity of Bacopa monniera on vascular and intestinal smooth muscles of rabbit and guinea-pig. Journal of Ethnopharmacology, 66, pp. 167-174.

Das A., (2002). A comparative study in rodents of standardized extracts of Bacopa monniera and Ginkgo biloba. Anticholinesterase and cognitive enhancing activities, Pharmacology, Biochemistry and Behavior, 73, pp. 893-900.

Trương Hoàng Duy, Lê Phạm Tấn Quốc, Trần Thị Hồng Cẩm, Phạm Thị Kim Ngọc, và Đống Thị Anh Đào (2015). Tối ưu hóa trích ly thu nhận dịch saponin thô từ Đẳng sâm Codonopsis Javanica (Blume) Hook.F. bằng enzyme alpha-amylase", Đặc san thông tin khoa học và công nghệ, 4(99), tr.1-3.

Fawzya Y.N., Putri S. and Noriko N. (2013). Identification of SGS 1609 cellulolytic bacteria isolated from Sargassum spec. and characterization of the cellulase produced. Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, 8 (2), pp. 57-68.

Fleurence J. (1999). The enzymatic degradation of algal cell walls: A useful approach for improving protein accessibility. Journal of Applied Phycology, 11(3), pp. 313–314.

Garg S.K. and Neelakantan S. (1981). Effect of cultural factors on cellulase activity and protein production by Aspergillus terreus. Biotechnology and Bioengineering, 23 (7), pp. 1653-1659.

Hai T.C., Nam N.D., Hong Anh L. T., Vu T. A. and Man P. V. (2016). Enzyme Assisted Extraction of Polyphenols from the Old Tea Leaves. Journal of Nutrition and Health Sciences, 3 (4), pp. 1-6.

Phạm Hoàng Hộ (2000). Cây cỏ Việt Nam, quyển 2. Tp. Hồ Chí Minh, Nhà Xuất bản Trẻ.

Jon Gabrielsson, Nils-Olof Lindberg and Torbjo¨ rn Lundstedt (2002). Multivariate methods in pharmaceutical applications. Journal of chemometrics, 16, pp. 141-160.

Pawar R., Gopalakrishnan C. and Bhutani K.K. (2001). Dammarane triterpene saponin from Bacopa monniera as the superoxide Inhibitor in polymorphonuclear cells. Planta Medica, 67, pp. 752-754.

Qu, W.J., Pan, Z.L. and Ma, H.L. (2010). Extraction modeling and activities of antioxidants from pomegranate marc. Journal of Food Engineering, 99 (1), pp. 16-23.

Russo A. (2003). Nitricoxide-related toxicity in cultured astrocytes: Effect of Bacopa monniera. Life Sciences, 73, pp. 1517-1526.

Sairam K. (2001). Prophylactic and curative effects of Bacopa monniera in gastric ulcer models. Phytomedicine, 8, pp. 423-430.

Shin, BK., Park, HV. and Han IH., (2010). Enzymatic biotransformation of red ginseng and the compositional change of ginsenosides. Journal of the Korean Society for Applied Biological Chemistry, 53(5), pp. 553-558.

Singh K. H. and Dhawan N. B. (1997). Neuropsychopharmacological effets of the Ayurvedic nootropic Bacopa monniera Linn. (Brahmi). Indian Journal of Pharmacology, 29, pp. 359-365.

Sivarajan V. V. and Balachandran I. (1994). Ayurvedic drugs and their plant sources. NewDelhi: Oxford and IBH Publishing.

Stough C. (2001). The chronic effects of an extract of Bacopa monnier (Brahmi) on cognitive function in healthy human subjects. Psychopharmacology, 156, pp. 481-484.

Sumathi T. (2002). Alcoholic extract of 'Bacopa monniera' reduces the in vitro effects of morphine withdrawal in guinea-pig ileum. Journal of Ethnopharmacology, 82, pp. 75-81.

Sunwoo H. H., Kim C. T., Kim D. Y., Maeng J. S. and Cho C. W., (2013). Extraction of ginsenosides from fresh ginseng roots (Panax ginseng CA Meyer) using commercial enzymes and high hydrostatic pressure. Biotechnology letters, 35 (7), pp. 1017-1022.

Ngô Văn Thu (1990), Hóa học saponin, Trường Đại học Y dược Thành phố Hồ Chí Minh.

Yan Z., Cao X., Teng Y. and Zhang J. (2012). A Shortcut to the Optimization of Cellulase Production Using the Mutant Trichoderma reesei YC-108. Microbiol, 52 (4), pp. 670 - 675.