Study of purification and immune activity of fucoidan from Kappaphycus alvarezii

Le Thi My Ngoc * , Dinh Thi Huyen , Nguyen Thi Minh Chi , Nguyen Pham Cam Tien , & Hoang Thi Ngoc Nhon

* Correspondence: Le Thi My Ngoc (email: nhonhtn@hufi.edu.vn)

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Received: 07 Nov 2018
Revised: 17 Dec 2018
Accepted: 17 Dec 2018
Published: 15 Jan 2019
DOI: 10.58810/vhujs.6.3.2019.6350
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Lê, N. . T. M., Đinh, H. T., Nguyễn, C. T. M., Nguyễn, T. P. C., & Hoàng, N. T. N. (2019). Study of purification and immune activity of fucoidan from Kappaphycus alvarezii. Van Hien University Journal of Science 6(3), 112-119.
Issue
Volume 6 - Issue 3 (2019)
Section
Công nghệ thực phẩm

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Abstract

Kappaphycus alvarezii is a seaweed containing numerous carbohydrate with biological activities, especially fucoidan. Fucoidan is fucose polysaccharides (FCSPs) with diverse biological activities including antioxidant, anticoagulant, antiviral, antitumor, anti-inflammatory... and especially immune activity. In this study, fucoidan purification and immune acitivity from Kappaphycus alvarezii algae (collected at Dam Mon area, Khanh Hoa provine) were investigated. Fucoidan was purified from the extract by gel filtration chromatography, the content and the purity in fractions were determined. The molecular mass of fucoidan was determined by GPC and the immune activity of fucoidan was evaluated with MTT. The average molecular weight of low fucoidan was 4.2 kDa. Fucoidan has been shown to be effective in the treatment of several types of cancer that cause peripheral blood mononuclear hyperplasia Peripheral Blood Mononuclear Cell (PBMC), at a concentration of 250μg/ml exhibiting toxicity and toxic depressant activity.
Keywords: fucoidan, immune activity, Kappaphycus alvarezii, purification

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